جهت دسترسی به کاربرگه ی زیر، از این لینک استفاده کنید. http://dl.pgu.ac.ir/handle/Hannan/82330
Title: Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
Keywords: Science & Technology;Multidisciplinary Sciences;Science & Technology - Other Topics;RESONANCE ENERGY-TRANSFER;OBLIQUE PLANE MICROSCOPY;IN-VITRO MODEL;CARDIAC MYOCYTES;DRUG DISCOVERY;LIVING CELLS;3D;CULTURES;PROTEIN;FRET
Issue Date: 25-Nov-2016
1-Nov-2016
Publisher: Nature Publishing Group
Description: Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.
URI: https://dx.doi.org/10.1038/srep37777
Other Identifiers: 2045-2322
http://hdl.handle.net/10044/1/42274
N/A
EP/H03238X/1
BB/I023801/1
EP/K503733/1
BB/M006786/1
Type Of Material: OTHER
Appears in Collections:Physics

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