جهت دسترسی به کاربرگه ی زیر، از این لینک استفاده کنید. http://dl.pgu.ac.ir/handle/Hannan/66312
Title: Imaging tumor microscopic viscosity in vivo using molecular rotors
Keywords: Science & Technology;Multidisciplinary Sciences;Science & Technology - Other Topics;FLUORESCENCE CORRELATION SPECTROSCOPY;LIVE CELLS;PHOTODYNAMIC THERAPY;CELLULAR-VISCOSITY;PLASMA-MEMBRANES;PHASE-SEPARATION;INNER MEMBRANE;ACID) BRUSHES;LIVING CELLS;SINGLE-CELL
Issue Date: 15-Mar-2017
Publisher: Nature Publishing Group
Description: The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice. We find that viscosities recorded from single tumor cells in vivo correlate well with the in vitro values from the same cancer cell line. Importantly, our new method allows both imaging and dynamic monitoring of viscosity changes in real time in live animals and thus it is particularly suitable for diagnostics and monitoring of the progress of treatments that might be accompanied by changes in microscopic viscosity.
URI: http://dx.doi.org/10.1038/srep41097
Other Identifiers: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000392849100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
2045-2322
http://hdl.handle.net/10044/1/44735
EP/I003983/1
327334
UF140161
Type Of Material: OTHER
Appears in Collections:Chemistry

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