جهت دسترسی به کاربرگه ی زیر، از این لینک استفاده کنید. http://dl.pgu.ac.ir/handle/Hannan/48899
Title: Zebrafish Globin Switching Occurs in Two Developmental Stages and Is Controlled by the LCR
Keywords: Zebrafish;Erythropoiesis;Globin switching;Locus control region
Issue Date: 2012
Publisher: Elsevier BV
Description: Globin gene switching is a complex, highly regulated process allowing expression of distinct globin genes at specific developmental stages. Here, for the first time, we have characterized all of the zebrafish globins based on the completed genomic sequence. Two distinct chromosomal loci, termed major (chromosome 3) and minor (chromosome 12), harbor the globin genes containing α/β pairs in a 5′–3′ to 3′–5′ orientation. Both these loci share synteny with the mammalian α-globin locus. Zebrafish globin expression was assayed during development and demonstrated two globin switches, similar to human development. A conserved regulatory element, the locus control region (LCR), was revealed by analyzing DNase I hypersensitive sites, H3K4 trimethylation marks and GATA1 binding sites. Surprisingly, the position of these sites with relation to the globin genes is evolutionarily conserved, despite a lack of overall sequence conservation. Motifs within the zebrafish LCR include CACCC, GATA, and NFE2 sites, suggesting functional interactions with known transcription factors but not the same LCR architecture. Functional homology to the mammalian α-LCR MCS-R2 region was confirmed by robust and specific reporter expression in erythrocytes of transgenic zebrafish. Our studies provide a comprehensive characterization of the zebrafish globin loci and clarify the regulation of globin switching.;Stem Cell and Regenerative Biology
URI: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12582486
Other Identifiers: Ganis, Jared J., Nelson Hsia, Eirini Trompouki, Jill L.O. de Jong, Anthony DiBiase, Janelle S. Lambert, Zhiying Jia, et al. 2012. Zebrafish Globin Switching Occurs in Two Developmental Stages and Is Controlled by the LCR. Developmental Biology 366, no. 2: 185–194.
0012-1606
Type Of Material: OTHER
Appears in Collections:Faculty of Arts and Sciences

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