Please use this identifier to cite or link to this item: http://dl.pgu.ac.ir/handle/10023/9163
Title: Anthocyanins and their physiologically relevant metabolites alter the expression of IL-6 and VCAM-1 in CD40L and oxidized LDL challenged vascular endothelial cells
Keywords: Adhesion molecule;Cyanidin-3-glucoside;Cytokine;Inflammation;Metabolites;QD Chemistry;Food Science;Biotechnology;QD
Issue Date: 2-Jun-2015
Description: This study was supported by funding from the UK Biotechnology and Biological Sciences Research Council (BBSRC) and the BBSRC Diet and Health Research Industry Club (BBSRC-DRINC), including BB/H532059/1, BB/H004963/1, BB/I006028/1, BB/I005943/1 and BB/J004545/1. A.C. is a Royal Society Wolfson Research Merit Award Holder.;Scope : In vitro and in vivo studies suggest that dietary anthocyanins modulate cardiovascular disease risk; however, given anthocyanins extensive metabolism, it is likely that their degradation products and conjugated metabolites are responsible for this reported bioactivity. Methods and results : Human vascular endothelial cells were stimulated with either oxidized LDL (oxLDL) or cluster of differentiation 40 ligand (CD40L) and cotreated with cyanidin-3-glucoside and 11 of its recently identified metabolites, at 0.1, 1, and 10 μM concentrations. Protein and gene expression of IL-6 and VCAM-1 was quantified by ELISA and RT-qPCR. In oxLDL-stimulated cells the parent anthocyanin had no effect on IL-6 production, whereas numerous anthocyanin metabolites significantly reduced IL-6 protein levels; phase II conjugates of protocatechuic acid produced the greatest effects (>75% reduction, p ≤ 0.05). In CD40L-stimulated cells the anthocyanin and its phase II metabolites reduced IL-6 protein production, where protocatechuic acid-4-sulfate induced the greatest reduction (>96% reduction, p ≤ 0.03). Similarly, the anthocyanin and its metabolites reduced VCAM-1 protein production, with ferulic acid producing the greatest effect (>65% reduction, p ≤ 0.04). Conclusion : These novel data provide evidence to suggest that anthocyanin metabolites are bioactive at physiologically relevant concentrations and have the potential to modulate cardiovascular disease progression by altering the expression of inflammatory mediators.;Publisher PDF;Peer reviewed
URI: http://www.scopus.com/inward/record.url?scp=84930178829&partnerID=8YFLogxK
http://dx.doi.org/10.1002/mnfr.201400803
Other Identifiers: Amin , H P , Czank , C , Raheem , S , Zhang , Q , Botting , N P , Cassidy , A & Kay , C D 2015 , ' Anthocyanins and their physiologically relevant metabolites alter the expression of IL-6 and VCAM-1 in CD40L and oxidized LDL challenged vascular endothelial cells ' Molecular Nutrition and Food Research , vol 59 , no. 6 , pp. 1095-1106 . DOI: 10.1002/mnfr.201400803
1613-4125
PURE: 244398503
PURE UUID: 4d6d6120-b085-42eb-9ae1-3225e218caae
Scopus: 84930178829
Type Of Material: OTHER
Appears in Collections:Chemistry (School of)

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